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Step-by-Step Guide to Mass Culturing Bobor Biofertilizer

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Introduction

In this video, fourth-year students from Mam Institute of Agriculture and Technology demonstrate the process of mass culturing Bobor biofertilizer as part of their AGM 451 course on bioinoculant production technology. For a deeper understanding of bioinoculants, you may refer to the Comprehensive Overview of Biotechnology and Its Applications.

Step-by-Step Process

  1. Sterilization of Equipment

    • Sterilize all equipment sequentially, including:
      • Carrier material
      • Acetobacter culture broth
      • Ethanol
      • Mixing tray
      • Polythene bags
    • Ensure hands are properly sterilized with ethanol.
  2. Preparation of Carrier Material

  3. Mixing the Bioinoculant

    • Add 250 ml of broth to the carrier material, using Worksman number 77 medium for the preparation of exoa bioinoculant.
    • Mix the components either manually or mechanically, ensuring the consistency allows for easy ball formation without breaking.
  4. Packaging

    • Pack the bioinoculant in 250 g quantities into polythene bags.
    • Seal the bags using an electric sealer.
    • Label each packet appropriately.

Conclusion

This process ensures the effective production of Bobor biofertilizer, contributing to sustainable agricultural practices. For best practices in crop care, consider reviewing the Essential Crop Care and Maintenance Practices for Grade 7 Agriculture and Fishery Arts.

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