Comprehensive Guide to Organic Acid Testing with Mosaic Diagnostics

[Music] Welcome to our webinar series for professionals

the event has started our first uh four hour a webinar event it's the first time I'm having three presenters on in one uh

event so I'm very excited I hope I can work out the technique and the people in and out

So today we're gonna have a focused on test from and they're called now Mosaic Diagnostics they used to be called Great

Planes but they have changed name and also their website has been launched actually today with uh under Mosaic

Diagnostics so all very exciting and new thinking Etc from um yeah previous Great Planes

Laboratories so and today is in the name of oat it nearly sounds like some religious thing that I'm talking about

um but where Jasmine Brown a naturopathic doctor will do an introduction to uh the tests

um to the oath test today and then in about 50 minutes we will have a break like a water in water outbreak

um and then we will present the next uh speaker which is a Dr vula that will go more into details about candida and

clusterity here and then he will present for two hours and then we might have a short water in water outbreak in the

middle of that as well before we have q a at the end and of course we will have q and E with Jasmine as well

so um and then at the end of that uh so after Dr Villa we have

um didn't say Goddard that is coming on uh with a lot more in details around oxalate and health so all very exciting

day and we will of course record the whole event and share the sessions with you I will try and cut it up so that we

have three separate sessions when we send it out to you so that you don't have to sit and fast forward if there's

something that you don't that you're not interested in so yeah but uh yeah apart from that you

most of you already know me I'm and Catherine um failment co-founder of Nordic

laborious and DNA live where we are just dedicated and focused on helping people to optimize their health through

functional medicine laboratory tests food supplements and of course healthy lifestyle

and uh but first presender is Dr Jasmine Brown who's a naturopathic doctor and she just loves everything to do with

education and she's working for Mosaic and does support and helps the Education team there so um and she did a

presentation with us a few weeks ago and it was very good and well attended so um yeah we have we have we have high high

hopes here Dr Brown sorry to put a little bit of pressure on you but uh ah yeah exactly it will not be a problem at

all so if you have questions or just want to chat with your lovely colleagues do that in the chat box

um I will take note of any like questions that I feel is relevant and I will put them at the end of Dr Brown's

presentation for the Q a um so that's kind of the the strategy for today's event so Jasmine please uh

unmute yourself and get your slides up on the screen and let's get this go rolling all right thank you and

Catherine for that introduction all right let me bear with me guys

all right we see it okay okay I'm gonna put you guys down

all right so thank you again for having me um I'm like Ann Catherine said I'm all

about education and um I'm just really grateful to be able to um go through just a introduction of our organic acid

test if you guys are with me I'm a few weeks ago this might sound familiar but that's okay this is information that um

you really do want to hear kind of on repeat um so I'm always glad to give this

presentation um so let's Jump Right In um as Ann Catherine said I am a naturopathic

doctor and one of the clinical Educators with Mosaic Diagnostics and I've been interpreting this test uh for the last

three years our objectives for today are first to discuss the importance of organic acid

testing um describing who could benefit understanding some of the most common

conditions supported by this profile I'll then dive deeper into a majority of the analytes that we are testing and

then I'll wrap up with some other profiles to consider um once you you know crack your results

all right so our organic acids um well what are they right um these are acids that contain an

organic radical think of things like citric acid or acetyl acetic acid and through our profile what we're looking

at are the organic acids that are produced by living organisms including us humans bacteria and fungi

by understanding them we get a understanding of how the human body is responding metabolically and kind of

what's inside of us too and how is that functioning metabolically and how that's affecting us

now the origin of this test if you have children um you know that typically at Birth

there is that newborn screening test they will prick the heel of the infants to rule out rare inborn errors of

metabolism think like PKU maple syrup urine disease these rare conditions we're not processing amino acids

properly um and if these conditions are not treated properly we will start to see

potentially things like poor feeding seizures little to no weight gain nausea vomiting illness infection and in the

unfortunate cases death now this is our profile again it kind of want to think of it as a peek inside to

metabolic functionality okay now why should you even order success

um and why is it so valuable oh for one thing it's super easy to collect you just give your morning urine

right you can do this in any age group we also actually have a pediatric collection bag so for your non-potty

trained individuals you can still do this test you also get a wide range of information

there's 76 markers just from one earned sample I mean it's very useful because we're

looking at functionality no matter what stage of life you are in or what diagnosis we can get some information

about the body cellular and metabolic response and how we can best support them no matter what they're going

through now specific supports can now give us more we can give have more confidence in what

we're specifically giving based off of these analytes yeah

this also helps us to get some understanding of underlying causes so is there you know oxalate issue a fungal

issue just maybe just nutritional need we can really get a sense of that and then I always like to use this as a

first line test um but remember it can be done at any point

in the healing Journey now the way I like to think of this profile I'm always describe it as a road

map uh oftentimes someone comes into your office and you're like okay you have this laundry list of symptoms where

do I start this profile is a really great way to kind of get your bearings um it can confirm some of your clinical

suspicions but I will say don't go in with specific expectations remember it can also open new doors that maybe you

weren't thinking about and then again close up doors that you've either been going through repeatedly or you've been

thinking about going down that might be closed for you and even though sometimes that might be

um unexpected it can still be very helpful for your clients on some of the doors if

you will that can be opened are GI dysbiosis oxalates mitochondrial dysfunction there are transmitter

imbalance nutritional deficiency toxin exposure and genetics now our key areas of focus on this

profile are the intestinal microbial growth and I'm going to go through all these and work that for you so no

worries those oxalates are glycolytic and mitochondrial metabolites to help us to evaluate for energy production

dysfunction or functionality Twitter a chance in our section we then have pyrimidines and fatty acids which

give us understanding of folate metabolism and how well we're using fat nutritional markers just gives us that

kind of functional nutritional status then we have our indicators of detox one of my favorite sections

um and then those amino acids that's that uh section that is more tailored or

reminiscent of that newborn screening test that I mentioned earlier those metaboloids

all right so who would benefit who you're going to run this thing on right so just kind of a

overview that oat provides useful information about how well the individual's body is functioning if it

balances the function are revealed then these imbalances could be contributing to several disease processes Now by

addressing these things we can improve Associated symptoms and support overall health and well-being now diseases that

are associated with metabolic imbalance and nutritional inefficiency that we really see a lot of support with this

profile or anxiety depression obesity insulin resistance or diabetes autism GI dysbiosis fatigue and just you know your

person who wants to biohack or uh just overall optimize their health and well-being

now from my experience my three years the most common things that I see are well supported are fatigue anxiety

autism depression GI complaints pain especially chronic pain fertility issues Parkinson's cognitive decline OCD add

and autoimmune conviction now some of the less common diagnoses or conditions that I see but

still find this profile to be very helpful are going to be more of your dermatologic complaints like asthma or

psoriasis I've seen a few cases of chronic herpes and acne be well supported with this and then I have had

the occasional case of hallucinations um so that's just from my personal experience as an educator

now let's jump into the analytes that we're measuring our first section again is that intestinal microbial overgrowth

now we have three different sections fungal bacterial Equestria the fungal section we have a specific UV Candida

analyte we then have aspergated aspergillus excuse me a lights and one fusarium analyte

our bacterial section is non-specific and then we have a very unique claustria section these are specific toxins to

this this family of bacteria and just so you're aware they do different from your kind of traditional toxin a toxin B I

have diarrhea toxins but they are coming from the same organisms now this is that first section when you

first uh get your profile this is what you're going to see at the top of page one

the first marker you should dry your eyes to is number seven arabinets this is that specific candida marker now

there's no tag under it so sometimes what I'll do or have do practitioners do is just write it in

um just so when you go back you know the dryer is to it now our orange tagged markers as you can

see the aspergillus ones infuserium those are your molds specific markers

so definitely keep your eye out for those and as we kind of talk about mold

remember mold produces toxic byproducts called mycotoxins and we do have a micotox profile that helps us to get an

understanding of that lipotoxin excretion and load and these tests are complementary to one another

one you can actually run them on the same here in Sample so there's um you don't need to collect your hearing

twice it also gives you information about mold and microtoxins and functionality at the

same time so if you're running these things separately you're often times wasting time

um because you're going to find out oh there's Michael Jackson or oh there's mold growth but then you have to

recollect you have to wait to get it sent to the lab you then have to wait for results to be ready and then you can

start uh the rest of your treatment but if we're doing them together you get answers right away other than realizing

that these two really need to be done together because mold growth is different than mycotoxin exposure and

excretion you can definitely have either one simultaneously or apart and you just want to make sure what you're looking at

in each case so definitely consider doing those together now in 2019 the Great Plains laboratory

uh before we switched over to Mosaic I published this towels an article and this was uh by Dr Shaw and Dr Matthew

Pratt Hyatt it's called the biochemical markers in the urine associated with gastrointestinal mold overgrowth are

linked with elevated urinary mycotoxins in patients with suspected mold illness now this is important because this

compared elevations of micro toxins in urine to the organic acid mold related growth markers in the urine and as you

can see here the red is your bicotoxin positive participants and as you can see those

organic acids are much higher than in our mycotoxin positive individuals and then

the second graph is showing us the actual toxins that were positive and then this last one shows us the same

thing but in relation to more of that um tricarbolylic or that marker that is related to fusarium

really cool article um if you can it's pretty easy to find so

check it out it's a really good read okay so now we're going to look at our bacterial markers remember these are

non-specific so it doesn't give me anything like oh this is H pylori or this is klebsiella It's just bacteria so

it's really just a good kind of screening like hey should I move forward with further

um stool testing or other GI testing now a common question I get is this telling me there's sibo

okay so small intestinal bacterial overgrowth unfortunately we cannot diagnose sibo from this test because

we're looking at urine these metabolites can come from the small intestine the large intestine and there's no real way

to say this is definitely this fall in testing but sometimes as you're seeing is elevated they're having that feeling

of fullness or bloating nausea and vomiting diarrhea you can actually get some information that says hey these

things are overgrowing let's do that further sibo testing but remember this test cannot back into step

now the last section on page one is your clostridium markers again these are specific

toxins to clostridian species number 17 being specific to C diff that for crystal

now these toxins differ from Toxin and toxin these toxin a toxin detoxes are actually disrupted in the tight

junctions in the gut and that's what's causing that diarrhea that's completely different it'll look at that on stool

tests but these toxins are actually more neurologic toxins and that's because they come from

amino acids that also are needed to make our neurotransmitters hold that a little deeper into this but

basically at this point what you want to understand is these toxins and our neurotransmitters look very similar

and because of that they are blocky in enzyme called field communicator hydroxylase or dbh okay so

basically what happens the clostridia it takes the amino acids from our diet the amino acids are specifically taking are

you know alanine pyrosine tryptophan and these are the amino acid precursors to your dopamine norepinephine epinephrine

and two or serotonin what happens is they block that enzyme so your dopamine cannot become norepinephrine and

epinephrine and then we get this whole imbalance that we'll discuss a little later

but um I say all that to say that section those four markers are going to be very important for you to draw your

eyes to on every single oat that you have okay let's talk about those oxalates

so oscillates are crystal like structures as you can see in this picture they're like pointy

sharp so a lot of times you'll see pain and that's any kind of pain can be stomach pain headaches migraines uh

vaginal pain joint pain any type this is also commonly seen in the autistic population

um so definitely think about that if you have a autistic individual coming to you the three metabolites that we're looking

at are glycolic glyceric acids and then we want to make sure that we're evaluating these because they are

connected to candida overgrowth mold exposure they're also associated with the need for B6 and can lead to that

mitochondrial dysfunction so what we want to remember about oxalates is that they come from a

compound called glyoxally This is a main source of the formation of oxalates they're derived from amino acids and

fungal organisms and basically what happens is our human lactate dehydrogenase takes that compound and

converts it into those oxalate metabolites this also happens from a square bait so if you're taking massive

amounts of vitamin C you can induce this there's also some from glycine but it's only about one percent of the lysine

pools so not much um and then these precursors is how the

human body forms them internally okay now that candida connection here you'll see this

on page two of every organic acid test that you get basically what's happening is candida

overgrows it lysis or isocitrate and produces glyoxylate so glyoxally is in worked upon by that

LDH to become these oxalate metabolites now some good news about this if you have adequate B6 we can shunt that back

into that Glycine and remember that's only about one percent of the oxalate pools so it's really a save for

metabolite the grand scheme so B6 is a really great therapy option when you see high oxalates

[Music] now treatment considerations here I also get that question and do I need to do a

little oxalate diet um mild elevations so slightly out of that reference range if they're eating

massive amounts of like spinach Berry soy chocolate nuts the Apple ahead reduce those things

um if they have severe pain or severe autistic symptoms whatever their symptoms may be then you might want to

consider reducing those higher Foods now a value over 250 that is a time where um I would consider doing more of a low

oxalate diet um so you definitely want to take into account what symptoms you have how

severe they are and then how high that elevation is all of those factors will help you determine how restrictive you

might need to be and then there's that section on how it presents on the profile

okay so the next two sections that we're looking at are the glycolytic and mitochondrial amplites

and these analytes I always like to look at them as a whole picture so it's markers 22 through 32. now what it's

doing here we're evaluating the use of glucose via glycolysis for ATP production

we're also measuring urinary byprox as a corrupt cycle because it got medians then that last section and these three

evaluates the function of amino acid usage for energy metabolism okay elevations give us insight into this

function and the ability of the mitochondria to actually produce this energy if these metabolites are building

up and coming out of the urine that tells me it's the enzymes needed to actually convert these into the next

step of the process aren't quite supported the way that they need to be so that tells me that there is increased

metabolic demand whether that be from nutritional deficiency or clostridia or candida or toxins or oxalates and from

there we can then say hey we need to figure out what these factors are remove them and then also give those

nutritional supports now this is what that section looks at these first two are your glycolytic

lactic and pyruvic now these give you an insight into how well the body is actually using glucose

now if we go back to biochem and then you should usage of glucose we produce pyruvate

now pyruvate has two destinies either it's going to become acetyl COA via private dehydrogenase or lactic acid

via lactate dehydrate okay now if there is an oxygen rich situation meaning you're well oxygenated you have

enough hemoglobin you're going to go down this pyruvate dehydrogenase

and make acetyl-coa you're going to then go through the citric acid cycle and then the citric acid cycle

look you're gonna make two ATP here and then you're going to go into the electrode transfer chain and make a net

of 32 to 34 ATP this is a very efficient process now if there's any hiccups in the citric acid cycle or the electron

transport chain or pyruvate dehydrogenase pyruvate either builds up or your body says I have to make energy

somehow so it goes down to less efficient lactate and in this case you're only making a

two ATP 2 verses 32 to 34. that's a big difference so we always want to take a

look at these two markers and see how well are we using that pyruvant it is that one I guess

now when you look at this section the second uh analyte that you want to make sure

you're drawing your eyes to on this entire profile is 24 systemic acid this marker is uh such a godsend when it is

elevated because this is related to toxic exposure now I can remember early on in my career

working with this I had a organic acid test that only had this marker High everything else looked beautiful

and this was at like a 300 it was crazy it turned out this person was severely exposed to herbicides and pesticides and

that was really all that was wrong so this analyte with elevate it could be uh very talent and you always want to look

further for chemicals metals or mole toxins now

this is special not only because we know there's toxic burden but it's special because the enzyme that converts sacinic

acid into fumaric is vaccinating dehydrogenase and it's the only enzyme to produce participate

in two different parts of energy production so it participates in the Krebs cycle and the complex two of the

electronic transport chain so if this is blocked and you have all this acidic acid coming out you not only have

energy production cycle issues you have two areas so these people are usually very fatigued uh when they have a lot of

that specific coming out okay so our next section are those neurotransmitters uh this is definitely

a section you want to be patient with yourself and learning and understanding uh but what you want to remember this is

a overview of urinary um metabolites of neurotransmitter so it's not the actual neurotransmitter it's the

breakdown byproduct dopamine being that homo vanillic acid or hva norepinephrine epinephrine

vanilla mandelic acid or VMA and that serotonin being the five hydroxy endocetic acid or 5-hiaa

now symptoms will give you Clues and the values will give you Clues on what may be going on internally

so because we're looking at end products this doesn't necessarily tell you how much is in the body it tells you how

much came out so if you have a lot coming out we can imply that there's a lot going on inside

and there's a little coming out we can imply that there's a little coming on inside but depending on symptoms there

may be a lot of neurotransmitter and poor breakdown so you always want to ask those questions and you always want to

uh relate this to who you're looking at okay now we do have a special ratio there

hvavma ratio this evaluates the activity of that dopamine beta hydroxylus enzyme and then I mentioned with the clostridia

so the opposite is that the converts our dopamine into a norepinephrine epinephrine

that enzyme isn't working properly because it's blocked or there's a snip or

there's a lot of cofactor or whatever it may be you're going to see that ratio increase

because there's more hva to DNA now what does so we know that the clostria toxins Market

we also know that there are cofactors here those cofactors are copper and vitamin C so definitely take a vitamin C

marker all the organic acid tests that can be super helpful but also consider copper testing I see a

lot of people especially uh during the pandemic when it was you know much more um we're much more isolated lots of

people were taking zinc like crazy and unknowingly depleting themselves of copper throwing off this enzymatic

functionality so make sure you're checking serum coppers or you can consider our copper zinc profile that we

offer and then sometimes there's a snip in that dbh as well so if there's no

clusteria those cofactors are normal then you do want to consider genetic testing

[Music] now this is a depiction of that section okay so all right first you'll have your

hva your VMA and then there's that ratio that's what actually looks pretty good and then and on page seven this is a

really nice kind of breakdown of how that phenylalan and tyrosine are being used by these organisms and creating

these toxic byproducts so the way I like to think of it is dopamine for Crystal HP HPA all come from the same parents

you know alanine and because they come from the same parent they're kind of like cousins if

you will so they look very similar but they're different and so these toxins are actually blocking the receptor sites

on dbh so dopamine only could go down the other pathway of becoming hva and that's why it builds up and this is an

issue because it's neurotoxic um associated with things like autism OCD anxiety depression fatigue

Parkinson's cognitive decline so we can see a wide range of symptom presentations when we

have that elevator ratio [Music] now we have another ratio

that we look at actually I'll show you here on number 37 the hva doe pack this is a really nice ratio because this

gives us understanding of the catechol all methyl transferase enzyme or comd now what this enzyme does is it

inactivates our active catecholamines Okay so it breaks them down into those hvas and VMAs

and this bottom depiction shows us that other route dopamine can go okay so it can become no path and then from dopax

it's worked on by comt it becomes hva now invert ratio is high that HPA dual pack that tells me that comt is fast

it's breaking down dopamine very very well maybe a little too well if that ratio is low then that tells me

that Doe pack is building up right if you have a lot of dope pack not a lot of hva then this comt is sluggish two

things you'll think about you know our um cofactors like CME magnesium even lithium is required here

so that's definitely what you want to keep your eye out for as well so as you can see here uh kind of the

same thing I just mentioned you know it fully breaks down these neurotransmitters and then

increased activity will increase the ratio decreased activity will uh decrease the ratio

now the last couple of markers on this section are the quinolinic and kiner in it these are neurotoxic byproducts of

tryptophan excuse me tryptophan metabolism um planolinux production is usually

stimulated by immune activation viral load Lyme toxic burden and um the need for NAD

so that compound is needed in mitochondrial functionality and we don't have enough we'll upregulate this

process to make it so at the very least it's NAD or B3 higher in it now this is another

byproduct of tryptophan metabolism again it's usually only elevated in certain situations like inflammation and then

also it's commonly elevated B6 efficiency so make sure you're giving that be a sex when you see kindness

okay so let's move on to ketones and fatty acids your ketones remember these are

byproducts of beta oxidation or your primary process for metabolizing fatty acids

this occurs inside of the mitochondria and it can't metabolize all of your fatty acids so short medium and long

chain um but easily it's going to break down your short chain fatty acids now your

medium and longer chain require L-Carnitine for the carnitine shuttle so they can get into the mitochondria and

then these we can see this upregulated in normal situations like fasting so if you have

an intermittent faster or someone who's on a specific low carb or keto diet you'll also see this in calorie deficit

so think about picky eaters or an anorexia you can see this in diabetes the blood sugar metabolic dysregulation

you'll also see this in chronic diarrhea and vomiting so think about your um bulimic individuals you might see this

as well lastly and this is the most common but sometimes we'll see in a chronic large

fungal overgrowth which usually after these other things have been roll that okay now the other markers

here 45 49 are called dicarboxylic acid these are secondary metabolites these come from

Omega fatty acid oxidation okay so this is a process that happens outside of the mitochondria it's kind of like a backup

Sphere for beta oxidation so when the lead either is overwhelmed or we don't have enough carnitine or not enough

nutrients to run beta oxidation we have to deal with those fats somehow and we use a less efficient process outside of

the mitochondria and that's where the sidecarboxylic acids come from now this section is looking at the

mitochondrial function and usage of fat as an energy source and like I said it can be influenced by that diet

so here's that section the way it's depicted on the profile and then remember 43 and 44 are your actual

ketones now our nutrition analytes our pyrimidines are looking at folate we

analyze your sun thiamine and these are related to RNA and DNA synthesis and you need folate to synthesize these strands

so if these are elevated in the urine we know we don't have an escalate now the B12 B6 uh B5 B2 vitamin C CoQ10

and AC and biotin are also analyzed as you can see there's a couple that have asterisks that's important because those

markers are functional Partners I mean we're not actually looking at the vitamin itself

in the urine we're looking at a metabolite that's breakdown or production

is dependent upon that particular vitamin okay now you'll some people will forget this so I always

like to remind everyone that there is this note at the bottom of the page asterisk means a high value for this

marker May indicate a deficiency of this vitamin and that's when it's high okay so the Astra will always be there

now that I point out number 55. do you see this one how this was starting to creep upwards well that tells us that

there's something's going on with the production of CoQ10 so three hydroxy three methylglutaric

acid or more commonly known as H and G COA okay so this is the compound that is worked on by hmg-coa reductase

this enzyme helps us to make CoQ10 it also helps us to make cholesterol now if you're familiar with that drugs you know

that statins actually block that enzyme and that's how we reduce cholesterol right well

when this is blocked hmg-coa increases and that's why number 55.

when elevated tells us okay something's going on where we're not able to convert that and make Marco Q10 now this is also

important if you're working in a pediatric population because cholesterol is also typically affected here

um in those cases in a lower cholesterol has been associated with autism and speech delay so it's definitely

something that if you see that high or high normal go ahead give that CoQ10 But be sure you're looking at that total

cholesterol as well okay so one of my favorite is actually this is the indicator of detoxification

because this gives us an understanding of how well the body is actually processing out toxin

so the Pyro glutamic acid that one has an asterisk as well this is our functional marker for glutathion status

so the higher the elevation the more in need of glutathione your body is basically the faster your body is trying

to pump this antioxidant out now the two hydroxy butyric this is produced with homocysteine uses

cystothion beta acid-based pathway or CBS then we have erotic acid that gives us

understanding of how we're excuse me metabolizing ammonia and then the two hydroxy hyperic this is produced

by GI bacteria when overgrown or in the presence of aspartame integral salicylates so I'm thinking about like

diet soda sugar-free gum on the aspartame or solicitly it's like certain foods or um

aspirin and this is what that section looks like and then those asterisks you just have

those notes here at the bottom okay um now I'm going to point out specifically

to hydroxybutyric acid because at the Hub is homocysteine now hormone system

there it is okay so homocysteine can we know use the methylfolate uh the methyl B12 to become methionine that's how we

recycle it or we can use this CBS Pathway to produce cysteine all right because we know if

homocysteine Rises too much it's actually inflammatory so our body has two internal ways of regulating how much

home assistance available now in some cases you're going to see number 59 or that two hydroxybutyric

acid elevated because this person's not methylated they don't have that methyl folate or their mthr is effective or

they need that B12 or they need that t of G or B Team in other cases what you're going to see

is two hydroxycutyric acid being produced because homocysteine is being converted into cysteine

now the only time two hydroxibutoric acid is really produced is when we are trying to

make cysteine from homocysteine so when you see that we then know that the cysteine is being produced rather than

if you need that glutathione status so if you're seeing that elevated it's always good first line Next Step

is to go ahead and check that homocysteine because if it's too high we know that we have a methylation issue if

it's normal or low there's usually a need for glutathione support and then further toxin testing

okay so our amino acid metabolites remember this is that a section that's associated

with the newborn screening um these are measuring metabolites they're linked to the emperor errors of

metabolism including things like maple syrup urine disease bku elevations are usually

consistent with genetic dysfunction when I say elevations I mean like 50s and hundreds you're going to crazy and

embarrassed you're probably going to be a little freaked out when you first see it

um but that's where I uh us educators are here to kind of help walk you through that but this is not going to be

typical most people that have these conditions at least nowadays are going to be well supported

um I already know that they have that now what I do want to make clear this is not a measure of amino acid status

including intake or digestion okay you can see low values and vegans vegetarians omnivores carnivores

because we're not looking at intake or breakdown we're looking at once you've been taking these amino acids once

you've digest them can you actually use them right can you metabolize them and transform them into the next parts of

wherever your body is using these if these enzymes aren't working that's what is my tablets start to build up and they

are toxic byproducts that's like elevations maybe lead to nutrient need like um in particular 62

through 66 are looking at maple syrup urine disease type metabolites or arthrusive Branch chain amino acids and

that's a B1 dependent process so sometimes you'll see people slightly elevated or slightly off that low

um and it's like oh maybe it's a big one so it can be helpful there and then again the more you do this the more can

you become become on these markers and these numbers okay so some common things uh just

questions I get um Can the oath serve as a screening test for mold exposure so again no

um the oak tells you about growth of mycotoxology about toxic excretion you can't have these things together but you

can't have them separately so don't let that oat fool you if it comes up not telling you anything about mold

um and then also get that question our analyze 15 through 18 specific to toxogenic C diff again these are

different toxins okay um do the mitochondrial markers all of the excuse me evaluate any specific area

of the body not really um you know any cell that produces energy is going to have a mitochondria

so we can be getting we'll be getting information from all over the body but you always want to think about

mitochondrial Rich tissues so the heart the brain the nervous system the muscles these things are full of mitochondria so

that's where you'll be getting a bulk of your information um I also get where in the body or brain

are the neurotransmitters measured against the urine tests so it's a nice overview

um and then why are the elevations of the micro talks and now oh and the mold um

analytes again one is telling you about growth right so growth of an organism

and the other is telling you about toxin load so organisms are living growing thriving versus toxins that are

non-living metabolites so that's what it can be um mismatched if you will

um and then again I get the question to do amino acids tell me about protein status I mentioned this again because it

does not uh please don't let that confuse you okay now lastly we're going to talk about

some other profiles for you to consider I've kind of talked about some of these but

we'll just go back through them the micro talks GPL talks and glyphosate

mic attacks if you have mold markers of course look for those toxins if you have oxalates in the absence of candida look

for those mycotoxins and again best done at the same time as the organic acid test

now that GPL taxes are chemical profile it's really a nice profile that we offer if you have 24 so that's acidic acid I

mentioned make sure you're evaluating this test um because it's blocking that

succinating hydrogenase enzyme causing that marker to elevate the glyphosate so number 24 uh

definitely look for glyphosate as well but it's really important if you see resistant clusteria so if you're a

treating loss trivia with probiotics biocidein or other antimicrobials and it keeps popping up

look for life as because life is a like the way it works unfortunately is it targets a specific pathway

in um organisms and unfortunately claustralia doesn't have this pathway but our good

bacteria do so if you're constantly getting exposed to this from your air your water your food

your class Julius like having a party in your gut so you want to make sure that you're removing that factor

and we have heavy metals and DNA methylation heavy metals again if marker 24 is elevated you want to rule out

toxin and then 59 so you know remember that two hydroxy butyric acid we're breaking down that homocysteine if

you check that home assistant at home assistance hi you want to know what steps am I dealing with here you know is

it just MTHFR is it mtrr or some other more obscure um

snip so definitely consider our salivary DNA methylation panel all right so our takeaways

the oat is beneficial for any chronic disease patient or anyone really is just looking to

get help better health overall various symptoms disease processes can be supported by this profile

our common um analytes are our intestinal overgrowth oxalates mitochondria

neurotransmitters nutrition and detox and then our other tests that I want you to consider are mycotoxin testing GPL

talks Chemical Testing heavy metals and then of course that DNA methylation stem panel

yeah all right what questions do you have for me today thank you Dr Brown rotation

um just a few questions has come up um uh one is and that's of course a

challenge with with vitamin C supplementation and high oxalates uh in the urine so if the oxalic acid is high

is um is is high and would you have a low Vitamin C marker and how would you would you just take

Vitamin C or how would you handle that challenge yeah so my typical recommendation there

is if you can get in and food um that's just good practice anyway make

sure we're eating vitamin C through our food but if you're looking for the antioxidant support at higher amounts I

typically find if you say under 1500 a day milligrams it's usually okay you just want to make sure you're breaking

that up um I wouldn't give that whole dose at once so maybe like you know

break it up twice or three times a day okay and then another one comes up here from

Camila um if low iconic tick asset marker 28

is that an indication for something particular so we haven't found any clinical

significance to that academic being low um one thing I will say academic comes from the citric and the Krebs cycle is

also known as has citric acid Cycles so typically what I'm looking for with the low academic is also a lower citric that

usually tells me there's not enough fuel if you will for that cycle so it usually like recommend adding some citric acid

in those cases so then of course a big question in regards to Parkinson's I guess it's like

using the old test if that will be relevant uh or if it can help reverse and controlled

the situation but I guess it goes a little bit with all function but when I mean with wind function when we're

working with functional medicine we are not perceived dealing with a diagnosis we're dealing with biochemistry and we

are working with the foundation and the cells and the mitochondria and the energy production and the detoxification

and when we are working on that we may be able to to well at least do Health optimization if we can fix a disease I

yeah sometimes we can yeah it's really about optimizing um and there's certain patterns right that we

see with Parkinson's in particular I'm a student with clostridias you know with mycotoxin exposure I've seen it with you

know a combination of things so um it's really just kind of like I said don't go on with expectations but go in and

understanding what you're looking at and how to support those um dysfunctions and that oftentimes will

support the person that you're sitting in front of excellent thank you very much uh time is

up so it's actually um time for oh now now lots of questions are coming in

um I'm just looking at quite that quite specific and um I think

I think we have to take the break and I need to get uh Dr vula in as well so um Dr Brown I'm gonna say thank you very

much for your presentation um and we will record it and we will share it with you all so you can go in

and listen to it as well again later um we will keep it in VMS um so uh yeah just keep a look out look

and look out for your email so we're gonna have a break now so you can go and have some uh something to drink or go to

the bathroom Etc and then we will uh start again at uh in well I've got to say nine minutes

because I'm not gonna say cook making time because your guys are all over the place so we will start in nine minutes

uh with Dr vola's presentation so um yeah enjoy your break and see you soon

and uh Jasmine thank you very much yes thank you as well

um and you guys got some great speakers coming up so enjoy yeah we do have excellent speakers coming on I'm very

excited okay all right if you do not already have an account with us please contact our team and we

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